Establishment of a reference panel for the detection of anti-SARS-CoV antibodies.
Identifieur interne : 003714 ( Main/Exploration ); précédent : 003713; suivant : 003715Establishment of a reference panel for the detection of anti-SARS-CoV antibodies.
Auteurs : Chunyu Liu [République populaire de Chine] ; Xiuhua Li ; Chuntao Zhang ; Sihong Xu ; Yiming Shao ; Hui Zhuang ; Xiaoyan Che ; Yan Qiu ; Hongzhang Yin ; Defu Li ; Youchun WangSource :
- Biologicals : journal of the International Association of Biological Standardization [ 1045-1056 ] ; 2007.
Descripteurs français
- KwdFr :
- ADN viral (génétique), Anticorps antiviraux (sang), Antigènes viraux (sang), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Gènes viraux, Humains, Normes de référence, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Séquence nucléotidique, Test ELISA (), Test ELISA (normes), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : ADN viral, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Virus du SRAS.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS.
- normes : Test ELISA.
- sang : Anticorps antiviraux, Antigènes viraux.
- virologie : Syndrome respiratoire aigu sévère.
- Glycoprotéine de spicule des coronavirus, Gènes viraux, Humains, Normes de référence, Séquence nucléotidique, Test ELISA.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Antigens, Viral (blood), Base Sequence, DNA, Viral (genetics), Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (standards), Genes, Viral, Humans, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Reference Standards, SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Antigens, Viral.
- chemical , genetics : DNA, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- methods : Enzyme-Linked Immunosorbent Assay.
- standards : Enzyme-Linked Immunosorbent Assay.
- virology : Severe Acute Respiratory Syndrome.
- Base Sequence, Genes, Viral, Humans, Reference Standards, Spike Glycoprotein, Coronavirus.
Abstract
The immunological assays for detection of antibodies against SARS-CoV were developed in-house and some of them are available commercially. However, the antigens used in these assays differed. In order to validate the reliability of these assays, the standard panel should be established. In this study, we have expressed and purified severe acute respiratory syndrome (SARS) structural proteins and their fragments and developed indirect enzyme-linked immunosorbent assays (ELISAs) that detect antibodies against the SARS N, N(1), N(2), S(1), S(C), S(2), and M proteins as well as the human coronavirus OC43 and 229E N proteins. These assays were used to screen 58 samples from SARS convalescent patients, 40 serial serum specimens from patients at different phases of SARS infection, and 88 plasma specimens from normal blood donors. The samples from normal blood donors were also tested for antibodies against other respiratory virus. The representative samples were chosen to comprise a reference panel of SARS antibodies that may be used for the detection of SARS. The panel is composed of 25 positive samples, 25 negative samples, 7 diluted samples for anti-N antibody, 6 diluted samples for anti-S antibody, and one sample for validating precision. Comparison of detection results with different SARS antibody assays indicated that our panel should differentiate the specificity and sensitivity of different assays.
DOI: 10.1016/j.biologicals.2006.11.001
PubMed: 17261372
Affiliations:
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type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Xiuhua" sort="Li, Xiuhua" uniqKey="Li X" first="Xiuhua" last="Li">Xiuhua Li</name></author><author><name sortKey="Zhang, Chuntao" sort="Zhang, Chuntao" uniqKey="Zhang C" first="Chuntao" last="Zhang">Chuntao Zhang</name></author><author><name sortKey="Xu, Sihong" sort="Xu, Sihong" uniqKey="Xu S" first="Sihong" last="Xu">Sihong Xu</name></author><author><name sortKey="Shao, Yiming" sort="Shao, Yiming" uniqKey="Shao Y" first="Yiming" last="Shao">Yiming Shao</name></author><author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name></author><author><name sortKey="Che, Xiaoyan" sort="Che, Xiaoyan" uniqKey="Che X" first="Xiaoyan" last="Che">Xiaoyan Che</name></author><author><name sortKey="Qiu, Yan" sort="Qiu, Yan" uniqKey="Qiu Y" first="Yan" last="Qiu">Yan Qiu</name></author><author><name sortKey="Yin, Hongzhang" sort="Yin, Hongzhang" uniqKey="Yin H" first="Hongzhang" last="Yin">Hongzhang Yin</name></author><author><name sortKey="Li, Defu" sort="Li, Defu" uniqKey="Li D" first="Defu" last="Li">Defu Li</name></author><author><name sortKey="Wang, Youchun" sort="Wang, Youchun" uniqKey="Wang Y" first="Youchun" last="Wang">Youchun Wang</name></author></analytic><series><title level="j">Biologicals : journal of the International Association of Biological Standardization</title><idno type="ISSN">1045-1056</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Antigens, Viral (blood)</term><term>Base Sequence</term><term>DNA, Viral (genetics)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Enzyme-Linked Immunosorbent Assay (standards)</term><term>Genes, Viral</term><term>Humans</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Reference Standards</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>Anticorps antiviraux (sang)</term><term>Antigènes viraux (sang)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Gènes viraux</term><term>Humains</term><term>Normes de référence</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Séquence nucléotidique</term><term>Test ELISA ()</term><term>Test ELISA (normes)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Test ELISA</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Genes, Viral</term><term>Humans</term><term>Reference Standards</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term><term>Gènes viraux</term><term>Humains</term><term>Normes de référence</term><term>Séquence nucléotidique</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The immunological assays for detection of antibodies against SARS-CoV were developed in-house and some of them are available commercially. However, the antigens used in these assays differed. In order to validate the reliability of these assays, the standard panel should be established. In this study, we have expressed and purified severe acute respiratory syndrome (SARS) structural proteins and their fragments and developed indirect enzyme-linked immunosorbent assays (ELISAs) that detect antibodies against the SARS N, N(1), N(2), S(1), S(C), S(2), and M proteins as well as the human coronavirus OC43 and 229E N proteins. These assays were used to screen 58 samples from SARS convalescent patients, 40 serial serum specimens from patients at different phases of SARS infection, and 88 plasma specimens from normal blood donors. The samples from normal blood donors were also tested for antibodies against other respiratory virus. The representative samples were chosen to comprise a reference panel of SARS antibodies that may be used for the detection of SARS. The panel is composed of 25 positive samples, 25 negative samples, 7 diluted samples for anti-N antibody, 6 diluted samples for anti-S antibody, and one sample for validating precision. Comparison of detection results with different SARS antibody assays indicated that our panel should differentiate the specificity and sensitivity of different assays.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Che, Xiaoyan" sort="Che, Xiaoyan" uniqKey="Che X" first="Xiaoyan" last="Che">Xiaoyan Che</name><name sortKey="Li, Defu" sort="Li, Defu" uniqKey="Li D" first="Defu" last="Li">Defu Li</name><name sortKey="Li, Xiuhua" sort="Li, Xiuhua" uniqKey="Li X" first="Xiuhua" last="Li">Xiuhua Li</name><name sortKey="Qiu, Yan" sort="Qiu, Yan" uniqKey="Qiu Y" first="Yan" last="Qiu">Yan Qiu</name><name sortKey="Shao, Yiming" sort="Shao, Yiming" uniqKey="Shao Y" first="Yiming" last="Shao">Yiming Shao</name><name sortKey="Wang, Youchun" sort="Wang, Youchun" uniqKey="Wang Y" first="Youchun" last="Wang">Youchun Wang</name><name sortKey="Xu, Sihong" sort="Xu, Sihong" uniqKey="Xu S" first="Sihong" last="Xu">Sihong Xu</name><name sortKey="Yin, Hongzhang" sort="Yin, Hongzhang" uniqKey="Yin H" first="Hongzhang" last="Yin">Hongzhang Yin</name><name sortKey="Zhang, Chuntao" sort="Zhang, Chuntao" uniqKey="Zhang C" first="Chuntao" last="Zhang">Chuntao Zhang</name><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Chunyu" sort="Liu, Chunyu" uniqKey="Liu C" first="Chunyu" last="Liu">Chunyu Liu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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